Multispectral Imaging for Microchip Electrophoresis Enables Point-of-Care Newborn Hemoglobin Variant Screening

Authors

Ran An, Case Western Reserve UniversityFollow
Yuning Huang, Case Western Reserve University
Anne Rocheleau, Case Western Reserve University
Qiaochu Zhang, Case Western Reserve University
Yuncheng Man, Case Western Reserve University
Zoe Sekyonda, Case Western Reserve University
Amma Owusu-Ansah, Case Western Reserve University
Umut A. Gurkan, Case Western Reserve UniversityFollow

Author ORCID Identifier

Ran An

Yuncheng Man

Amma Owusu-Ansah

Umut A. Gurkan

Document Type

Article

Publication Date

12-1-2022

DOI

10.1016/j.heliyon.2022.e11778

Abstract

Hemoglobin (Hb) disorders affect nearly 7% of the world's population. Globally, around 400,000 babies are born annually with sickle cell disease (SCD), primarily in sub-Saharan Africa where morbidity and mortality rates are high. Screening, early diagnosis, and monitoring are not widely accessible due to technical challenges and cost. We hypothesized that multispectral imaging will allow sensitive hemoglobin variant identification in existing affordable paper-based Hb electrophoresis. To test this hypothesis, we developed the first integrated point-of-care multispectral Hb variant test: Gazelle-Multispectral. Here, we evaluated the accuracy of Gazelle-Multispectral for Hb variant newborn screening in 265 newborns with known hemoglobin variants including hemoglobin A (Hb A), hemoglobin F (Hb F), hemoglobin S (Hb S) and hemoglobin C (Hb C). Gazelle-Multispectral detected levels of Hb A, Hb F, Hb S, and Hb C/E/A2, demonstrated high correlations with the results reported by laboratory gold standard high performance liquid chromatography (HPLC) at Pearson Correlation Coefficient = 0.97, 0.97, 0.93, and 0.95. Gazelle-Multispectral demonstrated accuracy of 96.8% in subjects of 0–3 days, and 96.9% in newborns. The ability to obtain accurate results on newborn samples suggest that Gazelle-Multispectral can be suitable for large-scale newborn screening and for diagnosis of SCD in low resource settings.

Keywords

genetic hemoglobin disorders, multispectral imaging, newborn screening, point-of-care diagnostics, sickle cell disease

Publication Title

Heliyon

Volume

8

Issue

12

Grant

R44HL140739; R41HL151015; R01HL133574; 1U54HL143541; T32HL134622; K25HL159358; R41DK119048

Funder

National Heart, Lung, and Blood Institute (NHLBI); National Institute of Diabetes and Digestive and Kidney Diseases

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

An, Ran; Huang, Yuning; Rocheleau, Anne; Zhang, Qiaochu; Man, Yuncheng; Sekyonda, Zoe; Owusu-Ansah, Amma; and Gurkan, Umut A., "Multispectral Imaging for Microchip Electrophoresis Enables Point-of-Care Newborn Hemoglobin Variant Screening" (2022). Faculty Scholarship. 103.
https://commons.case.edu/facultyworks/103