Author ORCID Identifier

Robert W. Maitta

Document Type

Article

Publication Date

1-8-2014

Abstract

Circulating immature platelet fraction (IPF) reflects real-time thrombopoiesis and correlates with platelet recovery from thrombocytopenic presentations. To understand the dynamics of IPF in platelet transfusions, we quantified the %-IPF in single-donor platelet components (SDP) during prolonged storage. %-IPF significantly increased from baseline by day 5 post-donation. Absolute IPF counts (A-IPC) had similar significant increments. However, gamma-irradiation suppressed the increments of %-IPF and A-IPC by >50%. Ultrastructural analysis of SDP units at day 10 showed well preserved morphology of immature platelets. Our findings suggest that IPF might actively expand ex-vivo and may have a longer shelf life than their mature counterparts. Closer study of IPF may be of critical clinical importance for transfusion practices.

Keywords

platelets, specimen storage, blood transfusion, platelet aggregation, transmission electron microscopy, uranyl acetate staining, blood, hemorrhage

Publication Title

PLoS ONE

Volume

9

Issue

1

Funder

Office of Diversity and Inclusion, University Hospitals Case Medical Center

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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