Steady Increment of Immature Platelet Fraction is Suppressed by Irradiation in Single-Donor Platelet Components during Storage
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Circulating immature platelet fraction (IPF) reflects real-time thrombopoiesis and correlates with platelet recovery from thrombocytopenic presentations. To understand the dynamics of IPF in platelet transfusions, we quantified the %-IPF in single-donor platelet components (SDP) during prolonged storage. %-IPF significantly increased from baseline by day 5 post-donation. Absolute IPF counts (A-IPC) had similar significant increments. However, gamma-irradiation suppressed the increments of %-IPF and A-IPC by >50%. Ultrastructural analysis of SDP units at day 10 showed well preserved morphology of immature platelets. Our findings suggest that IPF might actively expand ex-vivo and may have a longer shelf life than their mature counterparts. Closer study of IPF may be of critical clinical importance for transfusion practices.
platelets, specimen storage, blood transfusion, platelet aggregation, transmission electron microscopy, uranyl acetate staining, blood, hemorrhage
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Hong H, Xiao W, Maitta RW (2014) Steady Increment of Immature Platelet Fraction Is Suppressed by Irradiation in Single-Donor Platelet Components during Storage. PLoS ONE 9(1): e85465. 10.1371/journal.pone.0085465