Author ORCID Identifier

https://orcid.org/0000-0002-0331-9960

Document Type

Article

Publication Date

1-22-2014

DOI

10.1038/srep03796

Publication Title

Scientific Reports

Volume

4

College/School

Case School of Engineering

College/School

School of Medicine

Grant

RO1A1081534; RO1AI093282; U54EB15408; R21AI087107; DAMD17-02-2-0006; W81XWH-07-2-0011; W81XWH-09-2-0001

Funder

W.H. Coulter Foundation Young Investigator Award; Center for Integration of Medicine and Innovative Technology (CIMIT), U.S. Army Medical Research Acquisition Activity Cooperative Agreements; Brigham and Women’s Hospital (BWH) Biomedical Research Institute Translatable Technologies & Care Innovation Award; Brigham Research Institute for the Bright Futures Award; U.S. Army Medical Research & Materiel Command (USAMRMC) and Telemedicine & Advanced Technology Research Center (TATRC),

Abstract

HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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