Author ORCID Identifier

https://orcid.org/0000-0002-6140-4346

Document Type

Article

Publication Date

11-4-2022

DOI

10.1038/s41598-022-21672-9

Publication Title

Scientific Reports

Volume

12

Issue

1

College/School

Case School of Engineering

Department/Center

Chemical & Biomolecular Engineering

Grant

USDA Award No. 2018-68011-28691 and NSF Award No. 2045033

Funder

United States Department of Agriculture and the National Science Foundation

Abstract

Surface-grafted elastin has found a wide range of uses such as sensing, tissue engineering and capture/release applications because of its ability to undergo stimuli-responsive phase transition. While various methods exist to control surface grafting in general, it is still difficult to control orientation as attachment occurs. This study investigates using an electric field as a new approach to control the surface-grafting of short elastin-like polypeptide (ELP). Characterization of ELP grafting to gold via quartz crystal microbalance with dissipation, atomic force microscopy and temperature ramping experiments revealed that the charge/hydrophobicity of the peptides, rearrangement kinetics and an applied electric field impacted the grafted morphology of ELP. Specifically, an ELP with a negative charge on the opposite end of the surface-binding moiety assembled in a more upright orientation, and a sufficient electric field pushed the charge away from the surface compared to when the same peptide was assembled in no electric field. In addition, this study demonstrated that assembling charged ELP in an applied electric field impacts transition behavior. Overall, this study reveals new strategies for achieving desirable and predictable surface properties of surface-bound ELP.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Comments

Data found in the main text of this manuscript can be found on Harvard Dataverse:

https://doi.org/10.7910/DVN/TGGCTT

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