Document Type
Article
Publication Date
9-3-2024
Manuscript Version
vor
Abstract
Excitation-contraction coupling in skeletal muscle myofibers depends upon Ca2+ release from the sarcoplasmic reticulum through the ryanodine receptor/Ca2+-release channel RyR1. The RyR1 contains ∼100 Cys thiols of which ∼30 comprise an allosteric network subject to posttranslational modification by S-nitrosylation, S-palmitoylation and S-oxidation. However, the role and function of these modifications is not understood. Although aberrant S-nitrosylation of multiple unidentified sites has been associated with dystrophic diseases, malignant hyperthermia and other myopathic syndromes, S-nitrosylation in physiological situations is reportedly specific to a single (1 of ∼100) Cys in RyR1, Cys3636 in a manner gated by pO2. Using mice expressing a form of RyR1 with a Cys3636→Ala point mutation to prevent S-nitrosylation at this site, we showed that Cys3636 was the principal target of endogenous S-nitrosylation during normal muscle function. The absence of Cys3636 S-nitrosylation suppressed stimulus-evoked Ca2+ release at physiological pO2 (at least in part by altering the regulation of RyR1 by Ca2+/calmodulin), eliminated pO2 coupling, and diminished skeletal myocyte contractility in vitro and measures of muscle strength in vivo. Furthermore, we found that abrogation of Cys3636 S-nitrosylation resulted in a developmental defect reflected in diminished myofiber diameter, altered fiber subtypes, and altered expression of genes implicated in muscle development and atrophy. Thus, our findings establish a physiological role for pO2-coupled S-nitrosylation of RyR1 in skeletal muscle contractility and development and provide foundation for future studies of RyR1 modifications in physiology and disease.
Keywords
excitation-contraction coupling, muscle hypoxia, nitric oxide, ryanodine receptor, S-nitrosothiol, sarcoplasmic reticulum
Publication Title
Biochemical and Biophysical Research Communications
Grant
RO1 HL0591130
Rights
© 2024 The Authors. Published by Elsevier Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Qi-An Sun, Zachary W. Grimmett, Douglas T. Hess, Lautaro G. Perez, Zhaoxia Qian, Ruchi Chaube, Nicholas M. Venetos, Bradley N. Plummer, Kenneth R. Laurita, Richard T. Premont, Jonathan S. Stamler, Physiological role for S-nitrosylation of RyR1 in skeletal muscle function and development, Biochemical and Biophysical Research Communications, Volume 723, 2024, 150163, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2024.150163.