Document Type
Article
Publication Date
9-4-2024
Abstract
We present a comprehensive guide to light-sheet microscopy (LSM) to assist scientists in navigating the practical implementation of this microscopy technique. Emphasizing the applicability of LSM to image both static microscale and nanoscale features, as well as diffusion dynamics, we present the fundamental concepts of microscopy, progressing through beam profile considerations, to image reconstruction. We outline key practical decisions in constructing a home-built system and provide insight into the alignment and calibration processes. We briefly discuss the conditions necessary for constructing a continuous 3D image and introduce our home-built code for data analysis. By providing this guide, we aim to alleviate the challenges associated with designing and constructing LSM systems and offer scientists new to LSM a valuable resource in navigating this complex field.
Keywords
image processing, optical imaging, super resolution microscopy, fluorescence microscopy, microscopy, proteins
Language
English
Publication Title
Journal of Applied Physics
Grant
R35GM142466
Rights
© Author(s) 2024. This is an Open Access work distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Kramer, S. N., Antarasen, J., Reinholt, C. R., & Kisley, L. (2024). A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell. Journal of Applied Physics, 136(9).
Manuscript Version
Final Publisher Version