Document Type
Article
Publication Date
7-13-2012
Abstract
Ion-exchange chromatography relies on electrostatic interactions between the adsorbent and the adsorbate and is used extensively in protein purification. Conventional ion-exchange chromatography uses ligands that are singly charged and randomly dispersed over the adsorbent, creating a heterogeneous distribution of potential adsorption sites. Clustered-charge ion exchangers exhibit higher affinity, capacity, and selectivity than their dispersed-charge counterparts of the same total charge density. In the present work, we monitored the transport behavior of an anionic protein near clustered-charge adsorbent surfaces using fluorescence correlation spectroscopy. We can resolve protein-free diffusion, hindered diffusion, and association with bare glass, agarose-coated, and agarose-clustered peptide surfaces, demonstrating that this method can be used to understand and ultimately optimize clustered-charge adsorbent and other surface interactions at the molecular scale. Copyright © 2012 John Wiley & Sons, Ltd.
Keywords
anion-exchange matrix, clustered-charge ion-exchange adsorbent, diffusion, ion-exchange chromatography, protein adsorption
Language
English
Publication Title
Journal of Molecular Recognition
Grant
R01GM094246
Rights
© 2012 John Wiley & Sons, Ltd. This is the peer reviewed accepted manuscript version of the article and may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Recommended Citation
Daniels, C.R., Kisley, L., Kim, H., Chen, W.-H., Poongavanam, M.-V., Reznik, C., Kourentzi, K., Willson, R.C. and Landes, C.F. (2012), Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents. J. Mol. Recognit., 25: 435-442. https://doi.org/10.1002/jmr.2206
Manuscript Version
Accepted Manuscript