Document Type
Article
Publication Date
4-30-2024
Abstract
In their ‘Matters Arising’ manuscript, Saurabh et al. discuss two issues related to single-molecule Förster resonance energy transfer (smFRET) experiments: the use of the Gaussian noise approximation and spectral crosstalk. Their arguments are based on simulations obtained with parameters that differ significantly from the typical conditions measured experimentally, and, thus, from the regime included in the original study (Götz et al.1). In addition, they make claims about our multi-lab blind study that we would like to rectify. In Table 1, we provide a list of specific statements made by Saurabh et al. with our respective explanations.
Keywords
fluorescence resonance energy transfer, single molecule biophysics
Language
English
Publication Title
Nature Communications
Grant
40578
Rights
© The Author(s) 2024. This is an Open Access work distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Götz, M., Barth, A., Bohr, S.S.R. et al. Reply to: On the statistical foundation of a recent single molecule FRET benchmark. Nat Commun 15, 3626 (2024). https://doi.org/10.1038/s41467-024-47734-2
Manuscript Version
Final Publisher Version