Document Type
Article
Publication Date
7-28-2018
Abstract
OBJECTIVES: In platelets, α-synuclein is important in calcium-dependent granule release. Notably, cells release α-synuclein in setting of cell damage or death. Therefore, we investigated α-synuclein levels in plasma of single donor platelet (SDP) units during storage. METHODS: Aliquots were obtained from same SDP units for 7 days from day of donation. Additionally, randomly sampled SDP units at same storage time points were also assayed by enzyme-linked immunosorbent assay. RESULTS: α-Synuclein in SDP plasma increased continuously over time at each assayed time point. Significant increases were measured on day 3 (11.7 ± 9.6 ng/mL, P = 0.025), day 5 (15.3 ± 5.9 ng/mL, P = 0.002), and highest on day 7 (23.7 ± 5.6 ng/mL, P < 0.0001) compared to day 0 (1.1 ± 0.8 ng/mL). Similar significant results were obtained in randomly sampled SDP units at same corresponding time points. Flow cytometry showed that platelets had strong expression of α-synuclein and lacked expression of other synucleins. CONCLUSIONS: Increases of α-synuclein during SDP storage is a steady and continuous process that increases with time. Our findings indicate that α-synuclein may represent a biomarker of platelet biological state during storage. Further research will be needed to determine how α-synuclein increases correlate with platelets' function.
Keywords
biomarker, platelets, senescence, storage, α-synuclein
Publication Title
European Journal of Haematology
Volume
101
Issue
5
First Page
630
Last Page
634
Grant
P0178; R01 AI034343
Funder
Office of Diversity and Inclusion, University Hospitals Cleveland Medical Center; National Institutes of Health (NIH)
Rights
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Recommended Citation
Stefaniuk CM, Hong H, Harding CV, Maitta RW. α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets. Eur J Haematol. 2018;101:630–634. https://doi.org/10.1111/ejh.13152
Comments
This is the peer reviewed version of the following article: Stefaniuk CM, Hong H, Harding CV, Maitta RW. α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets. Eur J Haematol. 2018;101:630–634. https://doi.org/10.1111/ejh.13152, which has been published in final form at https://doi.org/10.1111/ejh.13152.