Document Type
Article
Publication Date
7-3-2023
Abstract
Examination of DNA variation is central to understanding the function of mammalian cells, tissues, and whole bodies. Extraction of high‐quality DNA from cells and tissues is necessary for innumerable different experiments. We present protocols for the extraction of DNA from both fresh samples and formalin‐fixed tissue. Methods for extracting DNA have been standardized and streamlined over the past couple of decades and many extraction kits are available for a reasonable cost. In addition, many of the extraction procedures can also be automated for even higher throughput sample preparation.
Keywords
blood, DNA, formalin-fixed paraffin-embedded, human tissue, saliva
Publication Title
Current Protocols
Rights
© 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.
Recommended Citation
Miskimen, K. L. S., & Miron, P. L. (2023). Isolation of genomicDNA from mammalian cells and fixed tissue. Current Protocols, 3,e818. doi: 10.1002/cpz1.818