Author ORCID Identifier
Document Type
Article
Publication Date
11-1-2023
Abstract
Intracortical microelectrode arrays (MEAs) are used to record neural activity. However, their implantation initiates a neuroinflammatory cascade, involving the accumulation of reactive oxygen species, leading to interface failure. Here, we coated commercially-available MEAs with Mn(III)tetrakis(4-benzoic acid)porphyrin (MnTBAP), to mitigate oxidative stress. First, we assessed the in vitro cytotoxicity of modified sample substrates. Then, we implanted 36 rats with uncoated, MnTBAP-coated (“Coated”), or (3-Aminopropyl)triethoxysilane (APTES)-coated devices – an intermediate step in the coating process. We assessed electrode performance during the acute (1–5 weeks), sub-chronic (6–11 weeks), and chronic (12–16 weeks) phases after implantation. Three subsets of animals were euthanized at different time points to assess the acute, sub-chronic and chronic immunohistological responses. Results showed that MnTBAP coatings were not cytotoxic in vitro, and their implantation in vivo improved the proportion of electrodes during the sub-chronic and chronic phases; APTES coatings resulted in failure of the neural interface during the chronic phase. In addition, MnTBAP coatings improved the quality of the signal throughout the study and reduced the neuroinflammatory response around the implant as early as two weeks, an effect that remained consistent for months post-implantation. Together, these results suggest that MnTBAP coatings are a potentially useful modification to improve MEA reliability.
Keywords
intracortical microelectrodes, MnTBAP coating, neuroinflammation, oxidative stress, recording neural interfaces
Publication Title
Biomaterials
Rights
© 2023 The Authors. Published by Elsevier Inc.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Ana G. Hernandez-Reynoso, Brandon S. Sturgill, George F. Hoeferlin, Lindsey N. Druschel, Olivia K. Krebs, Dhariyat M. Menendez, Teresa T.D. Thai, Thomas J. Smith, Jonathan Duncan, Jichu Zhang, Gaurav Mittal, Rahul Radhakrishna, Mrudang Spandan Desai, Stuart F. Cogan, Joseph J. Pancrazio, Jeffrey R. Capadona. The effect of a Mn(III)tetrakis(4-benzoic acid)porphyrin (MnTBAP) coating on the chronic recording performance of planar silicon intracortical microelectrode arrays. Biomaterials, Volume 303, 2023, 122351. https://doi.org/10.1016/j.biomaterials.2023.122351