Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging
Document Type
Article
Publication Date
7-30-2024
Abstract
Correlation signal processing of optical three-dimensional (x, y, t) data can produce super-resolution images. The second-order cross-correlation function XC2 has been documented to produce super-resolution imaging with static and blinking emitters but not for diffusing emitters. Here, we both analytically and numerically demonstrate cross-correlation analysis for diffusing particles. We then expand our fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) analysis to use cross-correlation as a postprocessing computational technique to extract both dynamic and structural information on particle diffusion in nanoscale structures simultaneously. Cross-correlation maintains the same super-resolution as auto-correlation while also increasing the sampling rates to reduce aliasing for spatial information in both simulated and experimental data. Our work demonstrates how fcsSOFI with cross-correlation can be a powerful signal-processing tool to resolve the nanoscale dynamics and structure in samples relevant to biological and soft materials.
Keywords
diffusion, cross-correlation, auto-correlation, fluorescence correlation spectroscopy imaging, super-resolution optical fluctuation imaging
Language
English
Publication Title
Chemical and Biomedical Imaging
Grant
R35GM142466
Rights
© 2024 The Authors. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/BY-NC-ND/4.0/), which permits non-commercial copying and redistribution of the material in any medium or format, provided the original work is not changed in any way and is properly cited.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Antarasen, J., Wellnitz, B., Kramer, S. N., Chatterjee, S., & Kisley, L. (2024). Cross-correlation increases sampling in diffusion-based super-resolution optical fluctuation imaging. Chemical & Biomedical Imaging, 2(9), 640-650.
Manuscript Version
Final Publisher Version