Document Type
Article
Publication Date
12-1-2024
Abstract
The significance of easily detecting rare earth elements (REEs) has increased due to the growing demand for REEs. Addressing this need, we present an innovative electrochemical biosensor, focusing on cerium as a model REE. This biosensor utilizes a modified EF-hand loop peptide sequence, incorporating cysteine for covalent attachment to a gold working electrode and tyrosine as an electrochemically active amino acid. The sensor was designed such that binding to cerium induces a conformational change in the peptide, affecting tyrosine's proximity to the electrode surface, modulating the current. A calibration curve was generated from cyclic voltammetry current peaks at ~0.55–0.65 V versus a silver pseudo-reference electrode, with cerium concentrations ranging from 0 to 67 μM in artificial urine. The sensor exhibited a biologically relevant limit of detection of 35 μM and a sensitivity of −0.0024 ± 0.002 (μA μM)−1. These findings offer insights into designing peptide sequences for electrochemical biosensing.
Keywords
biosensor, cyclic voltammetry, electrochemically active amino acid, lanmodulin peptide, rare earth elements
Language
English
Publication Title
AIChE Journal
Grant
2045033
Rights
© The Author(s) 2024. This is an open access work distributed under the terms of the Creative Commons Attribution-Non-Commercial (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Recommended Citation
Asaei S, Verma G, Sinclair NS,Renner JN. Electrochemical biosensing of cerium with atyrosine-functionalized EF-hand loop peptide. AIChE J. 2024;70(12):e18620. doi:10.1002/aic.18620
Manuscript Version
Final Publisher Version