Document Type
Article
Publication Date
8-30-2019
Abstract
Cerium has a wide range of current and emerging applications, and the binding of cerium ions to solid substrates is important for cerium recovery, or in advanced material synthesis. In this study, we investigate the affinity of a surface-bound peptide derived from the EF-hand loop I of calmodulin for cerium (III) ions and compare the results to a scrambled control. Results obtained via quartz crystal microbalance with dissipation are used to estimate the dissociation constant between the EF-hand loop I peptide and cerium (III) ions (1.3 ± 0.1 μM), which is comparable with other dissociation constants measured for EF-hand peptides and cerium ions in solution reported in literature (0.95-5.8 μM). Circular dichroism also suggests that the peptide binds to cerium (III) ions in solution, and undergoes a secondary structural change upon binding. Overall, this study shows that EF-hand loop peptides are capable of binding cerium (III) ions in solution and when attached to a solid substrate.
Keywords
affinity peptide, cerium, calmodulin, EF-hand motif
Publication Title
Peptide Science
Grant
1739473; 2018-68011-28691
Funder
National Science Foundation (NSF); United States Department of Agriculture; Case Western Reserve University
Rights
This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library is prohibited.
Recommended Citation
Xu, MingYuan; Su, Zihang; and Renner, Julie N., "Characterization of Cerium (III) Ion Binding to Surface‐Immobilized EF‐Hand Loop I of Calmodulin" (2019). Faculty Scholarship. 58.
https://commons.case.edu/facultyworks/58
Comments
This is the peer reviewed version of the following article: Xu, MY, Su, Z, Renner, JN. Characterization of cerium ion binding to surface-immobilized EF-hand loop I of calmodulin. Peptide Science. 2019; 111:e24133, which has been published in final form at https://doi.org/10.1002/pep2.24133.